CHIR99021 is the most selective inhibitor of glycogen synthase kinase 3β (GSK3β, IC50 7nM) reported so far and it does not inhibit cyclin-dependent kinases (CDKs).

CHIR99021 has been shown in long term expansion of murine embryonic stem cells in conjunction with MEK/MAPK inhibitor PD184352 and fibroblast growth factor receptor (FGFR) inhibitor SU5402.

Product Citation

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Technical information:

Chemical Formula:		C22H18CI2N8
CAS #:		252917-06-9
Molecular Weight:		465.34
Purity:		>98%
Appearance:		Pale solid
Chemical Name:		6-((2-((4-(2,4-Dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)pyrimidin-2-yl)amino)ethyl)amino)nicotinonitrile
Solubility:		Up to 25 mM in DMSO

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Shipping Condition: The product is shipped in a glass vial at ambient temperature.Storage: For longer shelf life, store solid powder at 4^oC desiccated, or store DMSO solution at -20^oC.

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Reference:

1.	Ring D et al. (2003). Selective Glycogen Synthase Kinase 3 Inhibitors Potentiate Insulin Activation of Glucose Transport and Utilization In Vitro and In Vivo. Diabetes. 52: 3, 588-595.
2.	Finley D et al. (2004). Glycogen Synthase Kinase-3 regulates IGFBP-1 gene transcription through the Thymine-rich Insulin Response Element. BMC Mol. Biol. 5:15.
3.	Ying Q. et al. (2008) The ground state of embryonic stem cell self renewal. Nature. 453: 519-523.

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Other Information:

Product Specification (pdf)MSDS (pdf)

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