P. aeruginosa Topoisomerase IV

Pseudomonas aeruginosa topo IV is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.

The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.

All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.	Product	Price	Quantity
PAT4001	P. aeruginosa Topo IV100 U	£136
PAT4002	P. aeruginosa Topo IV500 U	£498

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These contain the enzyme and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of topo IV will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. 

Technical Documents

Cat No.	Product	Price	Quantity
PAD4001	P. aeruginosa Topo IV Decatenation Kit 1100 U of P. aeruginosa topo IV enzyme and 20 µg kDNA	£220
PAD4002	P. aeruginosa Topo IV Decatenation Kit 2500 U of P. aeruginosa Topo IV and 100 µg kDNA	£817

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