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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack |
| Description | Three enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine, Kynurenic acid and Quinolinic acid in serum samples. |
| Labels | CE |
| Format | 3 x 96-well plate |
| Samples | Serum |
| Minimum sample volume | 20-50 µL |
| Cross specie-reactivity | React with all species |
| Standard range | KYN: 100 - 10000 ng/mlKYNA: 1.40 - 74 ng/ml QA: 200 - 2000 µg/ml |
| Sensitivity | LoD KYN: 47.5 ng/mlLoD KYNA: 0.53 ng/ml LoD QA: 6 ng/ml |
| Specificity | No significant cross-reactivity was observed analogs for each kit. See product pages for L-Kynurenine ELISA, Kynurenic acid ELISA and Quinolinic acid ELISA. |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use - KYN kit, Instructions for use - KYNA kit, Instructions for use - QA kit |
Key steps
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation | KYN: Sample acylation (90 min) KYNA: Sample derivatization (90 min) QA: Sample acylation (120 min) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Instructions for use - KYN kit, Instructions for use - KYNA kit, Instructions for use - QA kit |
References
KYN ELISA citations :
- Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.
- Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.
- Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3
- Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4
Selected articles on Kynurenic acid:
- Pedraz-Petrozzi et al. Effects of inflammation on the kynurenine pathway in schizophrenia - a systematic review. J Neuroinflammation. 2020
- Achtyes et al. Inflammation and kynurenine pathway dysregulation in post-partum women with severe and suicidal depression. Brain Behav Immun. 2020
- Walczak et al. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci. 2019
- Lim et al. Kynurenine pathway metabolomics predicts and provides mechanistic insight into multiple sclerosis progression. Sci Rep. 2017
- Plitman et al. Kynurenic Acid in Schizophrenia: A Systematic Review and Meta-analysis. Schizophr Bull. 2017
- Wirthgen et al. Kynurenic Acid: The Janus-Faced Role of an Immunomodulatory Tryptophan Metabolite and Its Link to Pathological Conditions. Front Immunol. 2017
Selected articles on Quinolinic acid:
- Fertan et al. Effects of the Novel IDO Inhibitor DWG-1036 on the Behavior of Male and Female 3xTg-AD Mice. Front Pharmacol. 2019 Sep;24
- Pierozan et al. Synergistic Toxicity of the Neurometabolites Quinolinic Acid and Homocysteine in Cortical Neurons and Astrocytes: Implications in Alzheimer"s Disease. Neurotox Res. 2018 Jul;34
- Hernandez-Martinez et al. Quinolinic acid induces neuritogenesis in SH-SY5Y neuroblastoma cells independently of NMDA receptor activation. Eur J Neurosci. 2017 Mar;45
- Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13
- Guillemin GJ. Quinolinic acid, the inescapable neurotoxin. FEBS J. 2012 Apr;279
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