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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | 3 catecholamines ELISA kit - Urine/Plasma - Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Adrenaline, Noradrenaline and Dopamine levels in plasma and urine samples. |
| Labels | IVD, CE |
| Format | 3x96-well plate |
| Samples | Urine, Plasma |
| Sample volume | 10 µl urine, 300 µl plasma |
| Cross specie-reactivity | React with all species |
| Standard range | Epinephrine: 0 / 1 - 200 ng/ml Norepinephrine: 0 / 5 - 1 000 ng/ml Dopamine: 0 / 4.5 - 2 000 ng/ml |
| Sensitivity | Epinephrine: 0.3 ng/ml urine, 10 pg/ml plasma Norepinephrine: 1.5 ng/ml urine, 40 pg/ml plasma Dopamine: 4.5 ng/ml urine, 25 pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Dopamine, Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
| Sample preparation | Sample preparation, extraction and acylation (65min) |
| ELISA | Dopamine, Epinephrine, Norepinephrine antisera incubation (2H), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Product citation: Chennupati et al. Myogenic vasoconstriction requires G12/G13 and LARG to maintain local and systemic vascular resistance. Elife. 2019 Sep 24
- Product citation: Elmarakby et al. Increased arterial pressure in mice with overexpression of the ADHD candidate gene calcyon in forebrain. PLoS One. 2019 Feb 12
- Product citation: Boisvert et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1-Deleted Mice. Clin Sci (Lond). 2018 Jul 18;132(13)
- Kang SW et al. Renal kallikrein excretion and epigenetics in human acute kidney injury: expression, mechanisms and consequences. BMC Nephrol. 2011 Jun 16;12:27.
- Dundon CM & Rellini AH. Emotional states of love moderate the association between catecholamines and female sexual responses in the laboratory. J Sex Med. 2012 Oct;9(10):2617-30.
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