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商品描述
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Epinephrine-Norepinephrine ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only. |
| Format | 3 x 96-well plate |
| Samples | Any sample |
| Sample volume | 1 - 750 µl |
| Cross specie-reactivity | React with all species |
| Standard range | Epinephrine, Dopamine: 0 / 0.5 - 80 ng/ml; Norepinephrine: 0 / 0.2 - 32 ng/ml |
| Sensitivity | Epinephrine, Dopamine: 10 pg/ml Norepinephrine: 4 pg/ml |
| Specificity | No significant cross-reactivity was observed with Epinephrine, Norepinephrine and Dopamine analogs such as Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | Sample preparation 4 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
| ELISA | Dopamine, Epinephrine, Norepinephrine antisera overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Horckmans M et al. Gene deletion of P2Y4 receptor lowers exercise capacity and reduces myocardial hypertrophy with swimming exercise. Am J Physiol Heart Circ Physiol. 2012 Oct 1;303(7):H835-43.
- Schneeberger M et al. Deletion of miRNA processing enzyme Dicer in POMC-expressing cells leads to pituitary dysfunction, neurodegeneration and development of obesity. Molecular Metabolism. 2013 Apr.
- Ohlsson C et al. Estrogen receptor-α expression in neuronal cells affects bone mass. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):983-8.
- Vida G et al. α7-cholinergic receptor mediates vagal induction of splenic norepinephrine. J Immunol. 2011 Apr 1;186(7):4340-6.
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