Product Specifications:

Item# 1003-B: Biotin p24 HIV-1 IIIB

 Mass/Vial: 50µg                                                                                 

 Vol./Vial: 50ul,50ug protein                                                           

 Diluent: 50mM Dodium Phosphate, 10mM NaC1,pH7.5                      

 Stabilizer: BSA                                                                                 

 Preservative: 0.02%                                                                               

 Storage: 4°C                                                                            

 Stability: 12 months at 4°C

 Minimum Dilution: 1 : 1000

 Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA

 Description: Biotin conjugated to recombinant (baculovirus) HIV-1p24.

 Coupling Ratio: Approximately 10-20 moles of biotin per mole p24. 

 Specificity: Biotin p24 binds to anti-p24 monoclonal and polyclonal antibodies in Elisa and western Elisa as determined by strepavidin-AP/HRP assays.

 Biological Activity: Biotin p24 binds to solid phase anti-p24 pAb (oligoclonal) as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

 Application and Instructions for use

 Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents.  p24/Anti-p24 pAb capture ELISA may be performed in 1-10ng pAb in solid phase and 0.1-1ng of biotin-p24.