PRODUCT SPECIFICATIONSProduct # 15015: Recombinant Treponema pallidum 15kD Antigen(E.coli)SPECIFICATIONS                                             APPLICATIONSConcentration: 1mg/ml                                     ElisaMass/vial: 1mg                                                 Western ElisaDiluent: PBS, pH7.2.                                        DiagnosticsPurity : >95%Stabilizer: NonePreservative: NoneStorage: -75 o CStability: At least 12 months at -75 o C

DESCRIPTION: T. pallidium full length recombinant 15kD antigen produced in E.coli expression system.

PURIFICATION: T. pallidium 15kD antigen is purified by immune-affinity and ion-affinity chromatography methods to >95% purity as determined by SDS-PAGE, reduce.

SPECIFICITY: This protein binds murine monoclonal antibodies and T. pallidium converted humanpolyclonal antibodies in ELISA and Western ELISA. Used for diagnosis of syphilis.BIOLOGICAL ACTIVITY: Not determined.APPLICATION AND INSTRUCTIONS FOR USERecommended concentrations for use are approximate values. Dilute the antigen preparation at least 1-5ug/ml in PBSfor solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determinethe optimal concentration for use in specific applications.T.pallidium 15kD antigen antigen shows relatively strong binding specificity for specific human antibodies.ELISA and Western ELISA require protein in 50ng range depending on the nature and affinity of thesecondary detection reagent. T. pallidium converted human polyclonal antibodies yield titers of 1:2 000 orgreater at 100ng of immobilized protein under standard