Product Specifications:

Item# 20014: Recombinant T. cruzi (Chagas) Composite Antigen A13,B13,H49,1F8 Antigen  Baculo)

Concentration: See vial                                          

Mass/vial: See vial                                           

Volume/vial: See vial                                                  

Diluent: 20mM NaPO4, 10mM NaCl, 0.05% Sark, pH8 .                                                   

Purity: >95%                                                                                                    

Stabilizer: None                                                                        

Preservative:None                                                                     

Physical State: Frozen Liquid (-75°C), Lyophilized (RT)

Stability: 3 years 

Application: In-Vitro Diagnostics, Immunization.

Description: Recombinant T. cruzi (Chagas) combination A13, B13, H49 and 1F8 antigen expressed in the Baculovirus Expression System.

Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.

Approx. Molecular Weight: 84kD.

Specificity: This protein binds to T. cruzi (Chagas) converted  human serum polyclonal antibodies in ELISA and  Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.

Biological Activity: Not done

Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.