ITEM# 4006:  Recombinant HCV NS5b Protein

   SPECIFICATIONS                                                                

  Concentration:    see vial                                                   

  Mass/vial:            see vial                                                    

  Volume/vial:        see vial                                                 

  Diluent:              PBS, 10% Glycerol 

  Purity                  >95%                                           

 Stabilizer:           None                                                      

  Preservative:     None                                                    

  Storage:             -75^o C

  Stability:            At least 24 months at -75^o C

DESCRIPTION: HCV NS5b  DNA  sequence expressed as a fusion protein in the E. coli expression system. MW: approx. 78kD

APPLICATIONS: In -vitro HCV Diagnostics,Western Analysis.

PURIFICATION: HCV NS5b protein was purified by solvent extraction and preparative gel electrophoresis to >95% purity as determined by SDS-PAGE, reduced.

SPECIFICITY:  This protein binds to murine monoclonal antibodies specific for HCV NS5 protein.    NS5b protein shows strong binding to HCV converted human serum polyclonal antibodies.

BIOLOGICAL ACTIVITY:   Not determined.

APPLICATION AND INSTRUCTIONS FOR USE

 Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10  to 20-fold in PBS for solid phase antigen-antibody titration assays.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.