The antibody produces a strong postive labeling of LHRH / GnRH at dilution of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The above image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection.Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.

Photo Description: IHC image of staining for LNRH/GnRH in the rat median eminence. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bat, Carp, Catfish, Chick, Chicken, Clarias Batrachus (Catfish), Fish, Frog, Frog (Green Treefrog), Gerbil, Guinea Pig, Guinea Pig, Hagfish, Hamster, Human, Lizard, Mole Rat, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Octopus, Pig, Rat, Salamander, Snail, Snail (Bulla Gouldiana), Squid, Trout

Availability: In Stock

Alternate Names:  Gonadotropin-Releasing Hormone; Gonadorelin; Luliberin I; Gonadoliberin I; progonadoliberin-1; GRH, anti-LHRH

Gene Symbol:  GNRH1 RRID:  AB_572248Database Links:  

  Entrez Gene:103295055    Bat    Entrez Gene: 770134        Chick  Entrez Gene: 100135026   Frog  Entrez Gene: 100135531   Guinea Pig  Entrez Gene: 100774729    Hamster  Entrez Gene: 2796             Human  Entrez Gene: 101705387    Mole Rat  Entrez Gene: 613033         Monkey  Entrez Gene: 14714           Mouse  Entrez Gene: 397516         Pig  Entrez Gene: 25194           Rat  Entrez Gene: 100135917    Trout