Description | ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL. |
Catalog Number | IW-PA1001 |
Quantity | 9 ml |
Host | Rabbit |
Clone | Polyclonal |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human Abi-1, different from the related rat and mouse sequence by single amino acid. |
Purity | Immunogen affinity purified |
Conjugate | Unconjugated |
Species Reactivity | Human, mouse, rat. Not tested in other species. |
Positive Control | Rat brain and spleen, human intestine, Hela cell |
Cellular Localization | Cytoplasmic |
Form | Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. |
Storage | Store at 2-8 °C. Do not freeze. |
Applications | IHC-P: Proteinase K digestion is required on formalin fixed paraffin sections. IHC-Fr: Formalin or acetone fixed frozen section. ICC: Acetone fixed cells. |
Limitations | This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. |
Precautions | Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. |
References | 1.Tani, K.; Sato, S.; Sukezane, T.; Kojima, H.; Hirose, H.; Hanafusa, H.; Shishido, T. : Abl interactor 1 promotes tyrosine 296 phosphorylation of mammalian Enabled (Mena) by c-Abl kinase. J. Biol. Chem. 278: 21685-21692, 2003. 2. Taki, T.; Shibuya, N.; Taniwaki, M.; Hanada, R.; Morishita, K.; Bessho, F.; Yanagisawa, M.; Hayashi, Y. : ABI-1, a human homolog to mouse Abl-interactor 1, fuses the MLL gene in acute myeloid leukemia with t(10;11)(p11.2;q23). Blood 92: 1125-1130, 1998. |
