INTENDED USE

This Human Interleukin 10 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 10 (IL-10) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not to be used in diagnostic or therapeutic procedures.

INTRODUCTION

The mature human IL-10 is a protein with 160 amino acids and the functional IL-10 is a homodimer. IL-10 is expressed by a variety of cells, including mouse T cells (TH1, TH2, and Tr1 subsets), B cells derived from peripheral blood, tonsils or spleen, Epstein–Barr virus (EBV)-transformed B cell lines, Burkitt’s lymphoma, AIDS lymphomas, monocytes, placental trophoblasts, bronchial epithelial cells, and certain tumor cells including melanomas and carcinomas of various origin.

IL-10 plays an important role in inflammatory and immune responses. The biological activities of IL-10 include both immunosuppressive and immuno-stimulatory effects. For example, IL-10 can suppress the production of pro-inflammatory cytokines by monocytes and neutrophils, and down regulates the expression of activating and co-stimulatory molecules on monocytes and dendritic cells. IL-10 also can improve the growth of B cells and mast cells, and inhibit or enhance the activities of T cells depending on their activation conditions. The different polymorphisms in the IL-10 gene promoter have been demonstrated the association with both SIDS (sudden infant death syndrome) and sudden unexpected death due to infection.

Based on the functions of IL-10, it has been suggested that an IL-10 antagonist can be applied to boost anti-viral immunity against viruses such as EBV and that the IL-10 molecule itself may be used as an anti-inflammatory reagent.

This IL-10 ELISA is a ready-to-use 3.5-hour solid phase immunoassay capable of measuring IL-10 levels in cell culture supernatant, serum, plasma, and other biological fluids in the range of 0 to 1600 pg/mL. This assay has shown no cross-reactivity with other cytokines tested.