Description:Mouse monoclonal antibody to human hepatitis virus C (HCV) non-structural protein NS-3

Purification:Protein G affinity purified

Product Type:Primary antibody

Target Protein:Human hepatitis C virus (HCV) non-structural protein NS-3

Immunogen:Purified recombinant chimeric HCV Polyprotein (555 amino acid residues)

Fusion Myeloma:Sp2/0-Ag14

Specificity:mAb 281 reacts with recombinant NS-3 (residues 1252-1477 on HCV polyprotein), synthetic NS-3 (residues 1378-1458), and recombinant chimeric HCV polyprotein (60 kDa).

Species Reactivity:Human hepatitis C virus, others not tested

Cross-Reactivity:No cross reaction can be seen with recombinant core protein C + envelop protein M(residues 1-142), synthetic core protein C (residues 1-61), and synthetic NS-4a protein (residures 1689-1735).

Host / Isotype:Mouse, IgG1 Kappa

Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.2

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area:Virology

Background:

Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.

Application:

ELISA:Reacts with HCV NS-3.

Western Blot:mAb clone 281, when used at concentration of 0.1-1μg/mL will allow visualization of 100ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 Protein. No reaction can be seen with synthetic NS-3. Test was conducted on blots transferred from both reducing and non-reducing PAGE gel.

References:

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