Description:Mouse monoclonal antibody against DYKDDDDK tag

Product Type:Purified primary antibodies

Target Protein:DYKDDDDK (Flag) tagged recombinant protein

Immunogen:A synthetic peptide (DYKDDDDK) coupled to KLH

Fusion Myeloma:Sp2/0-Ag14

Specificity:This monoclonal antibody binds to DYKDDDDK-tagged recombinant protein.

Cross-Reacitvity:Not identified

Host / Isotype: Mouse, IgG1 Kappa

Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2 with 0.05% sodium azide

Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Aliquot and store at -20^oC for long term storage. Avoid repeated freeze and thaw cycles.

Research Area:Life science

Application:

Western blotting: 1:1,000.

Western blot analysis of 293 cells transfected with DYKDDDDK- tagged vectors at different concentration, Lane 4: Lowest concentration (0.1 μg/mL).

Immunoprecipitation and Immunostaining: 1:200.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.