Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
- Product No.WEC918Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.77pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 505 For more details, please contact local distributors!US$ 722 For more details, please contact local distributors!US$ 3249 For more details, please contact local distributors!US$ 6137 For more details, please contact local distributors!US$ 50540 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
This assay has high sensitivity and excellent specificity for detection of Wide-range Fibroblast Growth Factor 21 (FGF21).No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Recovery of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
Matrices listed below were spiked with certain level of recombinant Wide-range Fibroblast Growth Factor 21 (FGF21) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Fibroblast Growth Factor 21 (FGF21) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-103 | 97 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 78-99 | 91 |
Precision of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-97% | 88-96% | 99-105% | 88-96% |
| EDTA plasma(n=5) | 78-94% | 79-103% | 96-105% | 91-101% |
| heparin plasma(n=5) | 90-104% | 81-90% | 94-103% | 87-95% |
Stability of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the Wide-range ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Fibroblast Growth Factor 21 (FGF21) and unlabeled Wide-range Fibroblast Growth Factor 21 (FGF21) (Standards or samples) with the pre-coated antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) in the sample.
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