Active Tumor Necrosis Factor Alpha (TNFa)
DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
- Product No.APA133Mu61
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 280 For more details, please contact local distributors!US$ 700 For more details, please contact local distributors!US$ 1400 For more details, please contact local distributors!US$ 4200 For more details, please contact local distributors!US$ 10500 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Gene sequencing
- SDS-PAGE
- Figure. Western Blot; Sample: APA133Mu61; Antibody: PAA133Mu06
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Tumor Necrosis Factor Alpha (TNFa)
TNF-a, being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .<br/>Cell necrosis after incubation with TNF-a (10ng/mL) for 72h was shown in Figure 1.
The production of IL-1β after incubation with TNF-a (10ng/mL) for 8h is shown in Table 1.
USAGE of the Active Tumor Necrosis Factor Alpha (TNFa)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Tumor Necrosis Factor Alpha (TNFa)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Tumor Necrosis Factor Alpha (TNFa)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
BCA Protein Quantification KitMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| APA133Mu01 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. |
| APA133Mu61 | Active Tumor Necrosis Factor Alpha (TNFa) | Cell culture; Activity Assays. |
| RPA133Mu02 | Recombinant Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA133Mu61 | Eukaryotic Tumor Necrosis Factor Alpha (TNFa) | Positive Control; Immunogen; SDS-PAGE; WB. |
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| PAA133Mu01 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
| PAA133Mu02 | Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
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| LAA133Mu71 | Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC. |
| MAA133Mu21 | Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) | WB; IHC; ICC; IP. |
| SEA133Mu | ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA133Mu | Wide-range ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA133Mu | Mini Samples ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA133Mu | CLIA Kit for Tumor Necrosis Factor Alpha (TNFa) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA133Mu01 | ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa) | Main materials for "Do It (ELISA Kit) Yourself". |
