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商品描述
| Source | CHO cells | M.W. | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
| Reconstitution | Before reconstitution, abrief spin is recommend to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a desired concentration. | ||||
| Stability | The lyophilized protein isstable for at least one year if stored at -80 °C. Reconstituted protein is stable forat least four weeks at 4 °C, but should be stored in aliquots at -80°C for longer term. Avoidrepeated freeze and thaw. | ||||
| Purity | Greater than 95% by SDS-PAGE analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The IC50 ranges from 100 - 200 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. | ||||
| Country of Origin | USA | ||||
Frizzled-5 is one of the 10 mammalian Frizzled receptors for WNTproteins. The extracellular cycteine rich domain of Frizzleds bind toWNTs with nM affinity. When supplied as recombinant protein, thisextracellular domain of Frizzled-5 acts as a soluble receptor, blocking thebinding of WNTs to Frizzled on the cells.
StemRD’s Frizzled-5 extracellular domain protein is fused to theN-terminus of the Fc domain of human IgG1. This fusion allows stabilizationof the protein in vitro and in vivo and dimerization of the recombinant proteinrendering high-affinity binding to WNTs. A similar protein, Frizzled-8 Fc fusion, has been used byGenentech scientists to show anti-tumor effect of WNT blockade in animal models(DeAlmeida VI et al, Cancer Res. 2007, 67:5371).
Expression is from CHO cells cultured in serum-free and protein-freemedium. Purification of the fusionprotein from conditioned culture medium is performed using protein Achromatography.
Frizzled-5 Fc fusion, human recombinant
