Description
RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor that is purified by affinity chromatography from E. coli. This protein inhibits pancreatic-type ribonucleases, RNase A, B, and C by binding strongly to RNases in a noncompetitive mode at a 1:1 ratio. RNAok™ RNase Inhibitor does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Extensive quality control tests ensure RNAok™ RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.
Application
RT-PCR
cDNA Synthesis
in vitro transcription
Storage Buffer
20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, stabilizer, 50% (v/v) glycerol
Storage
-20°C for 24 months
RNAok™ RNase Inhibitor inhibits RNase A activity
Total HeLa RNA (1 µg) was incubated with 1U/µl of RNAok™ RNase Inhibitor and 0.4 ng RNase A for 30 min at 37°C. The result shows that RNAok™ RNase Inhibitor can protect RNA against 0.4 ng RNase A. (M: DM3100)
Contents
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Storage
-20°C for 24 months
Manual
Manual_RI1000_RNAok™ RNase Inhibitor
SDS
SDS_RI1000
Usage Recommendation
Add RNAok™ RNase Inhibitor to transcription, translation, and cDNA synthesis reactions at a final concentration of 1 Unit/µl.
[RP1000] ExcelRT™ Reverse Transcriptase
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
[TF1000] SMO-HiFi™ DNA Polymerase
5’→3’ DNA polymerase activity
3’→5’ exonuclease (proofreading) activity
High reaction rate—10 seconds/kb
High fidelity— 70 times higher than Taq polymerase
Generates blunt end amplicon
Thermo-stable for more than 10 hrs at 95°C.
[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)
High sensitivity and signal intensity
Compatible with fast PCR program
With smart blue contrast dye as a visual aid for reaction setup
Low background
High stability
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix
5’→3’ DNA polymerase activity
No detectable 3"→5" exonuclease (proofreading) activity
Generates PCR products with 3"-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
