Description
The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Features
High Specificity
High Sensitivity
With ROX Reference Dye
Storage
Protect from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
High Sensitivity
The amplification plot of real-time PCR with cDNA templates ranging from 76 fg to 20 ng in quantity, analyzed by using TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) for qPCR amplification.
High Efficiency
The standard curve of TQ2110 ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) was generated by using 4-fold dilution of total cDNA ranging from 76 fg to 20 ng.
Contents
Component | Volume | Cat. No |
ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) | 2 x 1 ml | TQ2110 (200 Rxn) |
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
Manual
Manual_TQ2110_ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
SDS
SDS_TQ2110
Flyer
SMOBIO’s Q-PCR Master Mix- Instrument Compatibility
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
Instrument Compatibility* | |||||
Applied Biosystems system: | |||||
5700, 7000, 7300, 7700, and 7900HT | Δ | V | Δ | V | V |
StepOne, StepOnePlus | Δ | V | Δ | V | V |
7500, 7500 Fast, QuantStudio, ViiA7 | Δ | Δ | Δ | Δ | Δ |
BioRad system: | |||||
CFX96 , CFX384 | V | V | V | V | V |
iQ5 | V | V | V | V | V |
Opticon, Opticon 2, Chromo 4 | V | V | V | V | V |
Cepheid system: | |||||
Smart Cycler | V | V | V | V | V |
Eppendorf system: | |||||
Mastercycler ep realplex | V | V | V | V | V |
Roche system: | |||||
LightCycler 480, Nano | V | V | V | V | V |
QIAgen system: | |||||
Rotor-Gene™ 3000, 6000, Q | V | V | V | V | V |
Agilent (Stratagene) system: | |||||
MX3000P, 3005P, 4000P | Δ | Δ | Δ | Δ | Δ |
Illumina system: | |||||
Eco | V | V | V | V | V |
Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.
Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore
Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328
Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells
Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056
PMCID: PMC5522218
Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031
PMCID: PMC5706436
Loss of endogenous estrogen increases cardiac toxicity by doxorubicin
Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong
Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146
ExcelTaq™ 2X Q-PCR Master Mix
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
