scarabgenomics/pSX2表达载体/P-3330

价格
面议
货号:P-3330
浏览量:127
品牌:scarabgenomics
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商品描述

Background

ScarabXpress®-2 system, our second-generation protein expression system, consists of the pSX2 expression plasmid and ANY Clean Genome® E. coli host strain. In side by side comparisons, the ScarabXpress®-2 system protein expression system gave up to 36x higher yields than standard expression vectors with wild-type E. coli strains

Figures

Figure 1. pSX2 Expression Plasmid Map. Figure 2. 3000-Fold Induction Range. IPTG Titration of pSX2-GFP, intensity was measured, replicates were averaged and graphed in average fluorescent units. Figure 3. ScarabXpress2 Produces ≤ 36x More Protein Than BL21(DE3). Figure 4. More Consistent Fermentations With ScarabXpress®-2

Specifications

Kit Components pSX2 Expression Vector, 10 μg Quality Control The vector is confirmed to be IS-free and for functionality. Storage Conditions Store at ≤ -70°C. Do not store in a frost-free freezer.

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Support

Product Manuals pSX2 Expression Vector

Patents & Disclaimers

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications. Clean Genome® is a registered trademark of Scarab Genomics, LLC.

scarabgenomics重组蛋白生产是生命科学中使用的最强大的技术之一。产生和纯化大量靶重组蛋白的能力使多种可能性成为可能,包括其用于诊断或疾病治疗或在工业过程中的用途。乍一看,重组蛋白的表达看起来直截了当。encoding将编码所需蛋白的DNA克隆到表达载体中启动子的下游。将该克隆导入宿主细胞,细胞的蛋白质合成机制产生所需的蛋白质。但是,实际上,蛋白质表达可能会非常具有挑战性,因为可能有很多因素影响该过程。例如,某些蛋白质可能具有蛋白酶活性,这也可以通过选择表达宿主来解决。一些蛋白质可能具有有害于宿主的活性。ScarabXpress-1和ScarabExpress-2Δ之间有什么区别ScarabXpress1由ScarabXpress®T7 lac组成宿主+含有T7启动子的载体,例如pET载体。ScarabXpress2由Scarab的pSX2表达载体 + ANY CleanGenome® 大肠杆菌宿主菌株组成。