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蚂蚁淘在线 / 品牌中心 / PromoCell / 促进细胞/癌细胞系培养基XF

促进细胞/癌细胞系培养基XF

价格
面议
货号：C-28077
浏览量：127
品牌：PromoCell
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商品描述

The PromoCell Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability.

Components:The PromoCell Cancer Cell Line Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium

Note: Culture vessels need to be coated. For coating we recommend using Human Fibronectin Solution (C-43060) or Vitronectin (C-69201).

Key features:

  • Suitable for long-term routine culture of adherently growing established human cancer cell lines
  • Compatible with most commonly used human cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

TissueTested Cell LinesCell Lines OriginRemarks
BrainBV2immortalized murine primary microglial cellsCoat with Fibronectin:1 µg/cm2
BreastMCF-7pleural effusion of metastatic human breast adenocarcinomaCoat with Fibronectin:1 µg/cm2
ColonHT-29human colon adenocarcinomaCoat with Vitronectin:0.5 µg/cm2
Connective tissueHT1080human fibrosarcomaCoat with Fibronectin:1 µg/cm2
LiverHepG2hepatocellular carcinoma of the human liverCoat with Vitronectin:0.5 µg/cm2
LungA-549human lung carcinomaCoat with Vitronectin:0.5 µg/cm2
ProstateLNCaPlymph node metastasis of human prostate adenocarcinoma3D culture in C-28070 is recommended
Peripheral bloodMV-4-11Human acute myelogenous leukemia (suspension)No coating required
Bone marrowKG-1Human acute myelogenous leukemia (suspension)No coating required
KidneyACHNHuman Renal Cell CarcinomaCoat with Fibronectin:1 µg/cm2
BrainA172Human GlioblastomaCoat with Fibronectin:1 µg/cm2
Skeletal muscleC2C12Mouse MyoblastsCoat with Fibronectin:1 µg/cm2
SkinB16-F10Mouse MelanomaCoat with Fibronectin:1 µg/cm2
Abelson murine leukemia virus-induced tumorRAW264.7Mouse MacrophageCoat with Fibronectin:1 µg/cm2
Brain, neuroectodermalNeuro-2aMouse NeuroblastomaCoat with Fibronectin:1 µg/cm2
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 1. This figure shows example growth curves of four frequently used cancer cell lines cultivated in Cancer Cell Line Medium XF (C-28077), a serum- and xeno-free culture system. All of them exhibited a constant proliferation rate. The cell lines were plated at 5,000 cells/cm2 in T25 flasks coated with appropriate attachment substrates. For HT1080, 1 µg/cm2 of human fibronectin was used, while A549, HT29, and HepG2 were seeded on 0.5 µg/cm2 of recombinant human vitronectin. The cells were subcultured for between four and seven days depending on the cell line’s proliferation rate. The cumulative population doublings of 10 subsequent passages are shown for each cell line.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
Figure 2. Phase-contrast images of various cell lines cultured in serum- and xeno-free Cancer Cell Line Medium XF (C-28077). The cell lines robustly maintained their typical morphological phenotypes while exhibiting faster proliferation rates that with conventional serum-containing culture systems. The magnifications of the images shown are A: 100x, B: 40x, C: 100x, D: 40x, E: 100x, and F: 100x.
国PromoCell是细胞及细胞培养产品的主要制造商，公司主要致力于提供各种正常人类原代细胞、干细胞和血细胞制品、细胞培养的无血清无蛋白培养基、各类细胞培养液、细胞生长因子及无血清细胞冻存液等。此外， PromoCell还提供大量成熟的细胞生物学科研产品，例如细胞分析试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISA和细胞因子，Promocell还提供各类抗体、分子生物学相关试剂、检测试剂盒。Promocell所研发的各种相关产品，几乎覆盖细胞研究的所有领域，已被全球著名制药企业和研究院校的科研人员广泛使用。现部分产品现货供应，数量不多，先到先得。