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蚂蚁淘在线 / 品牌中心 / PromoCell / PromoCell/3D肿瘤球培养基XF

PromoCell/3D肿瘤球培养基XF

价格
面议
货号：C-28070
浏览量：127
品牌：PromoCell
服务
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商品描述

The PromoCell 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.

Components:

The PromoCell 3D Tumorsphere Medium XF consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.

Key features:

  • Establish 3D tumorsphere cultures directly from 2D cancer cell lines
  • Suitable for long-term routine culture of tumorspheres
  • Compatible with most commonly used cancer cell lines
  • Xeno-free and serum-free formulation

List of cell types tested for serial passage with the PromoCell 3D Tumorsphere Medium XF:

TissueTested Cell LinesCell Lines Origin
BrainU-87 MGgrade IV glioblastoma / astrocytoma of the human brain
BreastMCF-7pleural effusion of metastatic human breast adenocarcinoma
BreastMDA-MB-231pleural effusion of metastatic human breast adenocarcinoma (triple-negative)
ColonHT-29human colon adenocarcinoma
Connective tissueHT1080human fibrosarcoma
LiverHepG2hepatocellular carcinoma of the human liver
LungA-549human lung carcinoma
PancreasPanc-1epithelioid carcinoma of the human pancreatic duct
ProstateLNCaPlymph node metastasis of human prostate adenocarcinoma
SkinA-431epidermoid carcinoma of the human skin
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
Figure 1. HT1080 fibrosarcoma cells after 10 serial passages.
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
Figure 2. Proliferation curves of various established cancer cell lines expanded by serial passage of 3D tumorsphere cultures.
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.
Figure 3. Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in a significant increase of TFE from 2% in P1 to 28% in P9.
Figure 4. List of some cell types successfully tested for serial passage with the PromoCell 3D Tumorsphere Medium XF.
Figure 4. List of some cell types successfully tested for serial passage with the PromoCell 3D Tumorsphere Medium XF.
国PromoCell是细胞及细胞培养产品的主要制造商，公司主要致力于提供各种正常人类原代细胞、干细胞和血细胞制品、细胞培养的无血清无蛋白培养基、各类细胞培养液、细胞生长因子及无血清细胞冻存液等。此外， PromoCell还提供大量成熟的细胞生物学科研产品，例如细胞分析试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISA和细胞因子，Promocell还提供各类抗体、分子生物学相关试剂、检测试剂盒。Promocell所研发的各种相关产品，几乎覆盖细胞研究的所有领域，已被全球著名制药企业和研究院校的科研人员广泛使用。现部分产品现货供应，数量不多，先到先得。