The PromoKine 3D Cell Culture Harvesting Kit provides an optimized and standardized saline-based solution for a simplified non-enzymatic isolation of cells and spheroids from matrices (especially for the PromoKine 3D Cell Culture Kits) with high viability rates for subsequent biochemical, protein and cell-based analysis. This kit provides reagents for extracting cells and/or cell aggregates from 3D cell cultures for biochemical analysis, as well as reagents for the preparation of lysates for immunoblotting.