联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述

PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

NEW: Our hMNC are now also available from HLA-typed donors.

Available formats:

  • Cryopreserved: Cryogenic vial containing 25.000.000 viable cells.
  • Cell pellet: 1 million cells dissolved in 200µl RNAlater© for subsequent RNA, DNA or protein analysis. Cell pellets cannot be revived.
Recommended plating density1,000,000 cells per ml
Tested MarkersCD31 negative, CD34 negative, CD105 positive, CD146 positive
Figure 1. Flow cytometric analysis of Mononuclear Cells from Cord Blood (hMNC-CB). Granulocytes content 1%.
Figure 1. Flow cytometric analysis of Mononuclear Cells from Cord Blood (hMNC-CB). Granulocytes content 1%.
Figure 2. Flow cytometric determination of CD34cells within the PromoCell Mononuclear Cells from Cord Blood (hMNC-CB)
Figure 2. Flow cytometric determination of CD34cells within the PromoCell Mononuclear Cells from Cord Blood (hMNC-CB)
国PromoCell是细胞及细胞培养产品的主要制造商,公司主要致力于提供各种正常人类原代细胞、干细胞和血细胞制品、细胞培养的无血清无蛋白培养基、各类细胞培养液、细胞生长因子及无血清细胞冻存液等。此外, PromoCell还提供大量成熟的细胞生物学科研产品,例如细胞分析试剂盒、细胞转染试剂、荧光染色剂、抗体、ELISA和细胞因子,Promocell还提供各类抗体、分子生物学相关试剂、检测试剂盒。Promocell所研发的各种相关产品,几乎覆盖细胞研究的所有领域,已被全球著名制药企业和研究院校的科研人员广泛使用。现部分产品现货供应,数量不多,先到先得。