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蚂蚁淘在线 / 品牌中心 / Biotium / 生物素/瑞苏林细胞活力测定试剂盒/30025-1/25毫升

生物素/瑞苏林细胞活力测定试剂盒/30025-1/25毫升

价格
￥2440.00
货号：30025-1
浏览量：127
品牌：Biotium
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商品描述
ResazurinCellViABIlityAssayoffersasimple,rapid,reliable,sensitive,safeandcost-effectivemeasurementofcellviability.Thiskitperformsatleastaswellasothercommercialresazurin-basedcellproliferationassaykitssuchasalamarBlue®orCellTiter-Blue®.

ResazurinCellViabilityAssayoffersasimple,rapid,reliable,sensitive,safeandcost-effectivemeasurementofcellviability.Thiskitperformsatleastaswellasothercommercialresazurin-basedcellproliferationassaykitssuchasalamarBlue®orCellTiter-Blue®.Resazurindetectscellviabilitybyconvertingfromanonfluorescentdyetothehighlyredfluorescentdyeresorufininresponsetochemicalreductionofgrowthmediumresultingfromcellgrowth(1-3).Continuedcellgrowthmaintainsareducedenvironmentwhileinhibitionofgrowthmaintainsanoxidizedenvironment.Reductionrelatedtogrowthcausestheredoxindicatortochangefromoxidized(nonfluorescent,blue)formtoreduced(fluorescent,red)form.Thefluorescentsignalismonitoredusing530-560nmexcitationwavelengthand590nmemissionwavelength.Theabsorbanceismonitoredat570nmand600nm.Thefluorescentorcolorimetricsignalgeneratedfromtheassayisproportionaltothenumberoflivingcellsinthesample.Resazurinsolutionissuppliedready-to-usein25mL(2500assay)or100mL(10,000assay)sizes.

Features:

  • Sensitive:Requiringasfewas80cellsforreproducIBLeresults
  • Simple:Single-stephomogenousassay
  • KineticMonitoring:Reagentsnontoxictocells,thusallowingkineticmonitoring.

alamarBlueisaregisteredtrademarkofTrekBiosystems.CellTiter-BlueisaregisteredtrademarkofPromegaCorporation.

References

1.Tottey,S.,etal.Extracellularmatrixdegradationproductsandlowoxygenconditionsenhancetheregenerativepotentialofperivascularstemcells.TissueEngineeringdoi:10.1089/ten.TEA.2010.0188(2010).

2.Kanagaraj,R.,etal.RECQhelicaseassociateswiththeC-terminalrepeatdomainofRNApolymeraseIIduringproductiveelongationphaseoftranscription.NucleicAcidsResearchdoi:10.1093/nar/gkq697(2010).

3.Gustafsson,A.,etal.TheantimicrobialpeptideLL37anditstruncatedderivativespotentiatesproinflammatorycytokineinductionbylipoteichoicacidinwholeblood.ScandanavianJ.ofClinical&Lab.Inves.DOI:10.3109/00365513.2010.521255(2010).

4.Muzumdar,R.,etal.AcuteHumaninTherapyAttenuatesMyocardialIschemiaandReperfusionInjuryinMice.Arteriosclerosis,Thrombiosis,andVascularBIOLOGyDOI:10.1161/ATVBAHA.110.205997(2010).

5.Tottey,S.,etal.Theeffectofsourceanimalageuponextracellularmatrixscaffoldproperties.Biomaterialsdoi:10.1016/j.biomaterials.2010.09.006(2010).

6.Sarker,S.,etal.Microtitreplate-basedantibacterialassayincorporatingresazurinasanindicatorofcellgrowth,anditsapplicationintheinvitroantibacterialscreeningofphytochemicals.Methods42(2):321-24(2007).

7.Petrenko,Y.,etal.TheReductionofAlamarBluebyPeripheralBloodLymphocytesandIsolatedMitochondria.DOI612.112.94:576.311.347(2005).

8.Palomino,J.,etal.ResazurinMicrotiterAssayPlate:SimpleandInexpensiveMethodforDetectionofDrugResistanceinMycobacteriumtuberculosis.AntimicrobialAgentsandChemotherapyDOI:10.1128/AAC.46.8.2720

Biotium 5(6)-羧基-X-罗丹明-N-羟基琥珀酰亚胺酯 Biotium 异硫氰酸荧光素酯 Biotium 10-壬基吖啶橙溴化物 Biotium 4-甲基伞形酮磷酸二钠盐三水 Biotium 河豚毒素 Biotium 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐 Biotium N,N-二甲基甲酰胺 Biotium N-生物素酰基-L-赖氨酸酰肼 Biotium 5-溴-6-氯-3-吲哚基-beta-D-半乳糖苷 Biotium N,N,N',N'-四(2-吡啶甲基)乙二胺 Biotium 腔肠素-H Biotium Α-金环蛇毒 Biotium 5-溴-4-氯-3-吲哚半乳糖苷 Biotium BIOTIN-(AC5)2-OSU Biotium 4',6-二脒基-2-苯基吲哚二盐酸盐 Biotium 4-甲基伞形酮磷酸酯 Biotium 90% (TLC) Biotium 96314-98-6 SULFORHODAMINE G 5873-16-5 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 127770-45-0 HYDROETHIDINE 38483-26-0 SULFORHODAMINE 101 60311-02-6 L-N6-(1-IMINOETHYL)LYSINE DIHYDROCHLORIDE 150403-89-7