Overview
The E-Z 96® FastFilter Plasmid Kit is optimized for the parallel preparation of up to 96 plasmid minipreps in 1 hour. The Lysate Clearance Plate replaces the time-consuming centrifugation involved in clearing of bacterial alkaline lysates. This kit contains all of the components needed for plasmid DNA purification in a 96-well format. Purified plasmid DNA is suitable for the most sensitive downstream applications, such as routine screening or automated DNA sequencing. The E-Z 96® Fastfilter Plasmid Kits newly designed 96-well DNA plates are ideal for use with automated liquid handlers.
- Clear lysate with E-Z 96® Lysate Clearance Plate
- No resin, slurries, or alcohol precipitation
- High-purity plasmid DNA in less than 1 hour
- Reproducible yields of molecular biology grade plasmid DNA
- Fast, easy and convenient protocols
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specification |
|---|---|
| Downstream Application | Cloning; sanger sequencing etc. |
| Starting material | Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | 96-well, centrifugation or vacuum |
| Throughput | 96 samples per run |
| DNA binding technology | Silica 96-well plate |
| Lysate clearance method | Centrifugation or vacuum with lysate clearnce plate |
| Processing time | <1> |
| Yield | 15-25 µg for high copy-number; 0.1-5 µg for low copy-number |
| Special note | Lysate clearance plate for fast processing |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
D1097 E-Z 96 Fastfilter Plasmid
SDS
D1097 SDS
SALES SHEET
Product Data
Figure 1 Title
Figure 1. Plasmid DNA purified using the E-Z 96® FastFilter Plasmid Kit. DNA was isolated from 48 1 mL bacterial cultures frown overnight in LB culture using the E-Z 96® FastFilter Plasmid Kit and eluted in 150 µL elution buffer. Plasmid DNA (10% of total purified DNA) was analyzed on 1.0% agarose gel. Lanes 1-48 represent plasmid DNA from 48 different bacterial cultures.
Publications
- Long-term cathode performance and the microbial communities that develop in microbial fuel cells fed different fermentation endproducts Patrick D. Kiely, Geoffrey Rader, John M. Regan, Bruce E. Logan
- Enrichment of microbial electrolysis cell biocathodes from sediment microbial fuel cell bioanodes John M. Pisciotta, Zehra Zaybak, Douglas F. Call, Joo-Youn Nam, Bruce E. Logan
