Overview
The Mag-Bind® RxnPure Plus Kit allows rapid and reliable isolation DNA from PCR* and other enzymatic reactions with high yield. The system combines Omega Bio-tek’s proprietary DNA extraction chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively bind PCR amplicons 100 bp and larger and eliminate excess nucleotides, primers, and nonspecific products such as primer dimers.
This kit is designed for both manual and fully automated protocols and may not require reprogramming of liquid handling instruments depending on your current method.
The Mag-Bind® RxnPure Plus is compatible with multiple liquid handlers and magnetic processors such as Hamilton STAR/STARlet and Beckman Coulter Biomek FX/NX.
- Removes excess primers, primer-dimers, dNTPs and salts
- Does not require centrifugation/filtration steps
- Can be adapted and scaled to most standard liquid handling robots
- Available in 96- or 384- well format
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Special Note | Mag-Bind TotalPure NGS is updated version |
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 40 µL or above |
| Starting material | PCR products |
| Starting amount | Scalable |
| DNA recovered | >90% recovery for DNA >100bp |
| Processing mode | Automated; Manual |
| Throughput | 96-384 samples per run |
| DNA binding technology | Magnetic beads |
| Binding capacity | Scalable |
| Storage | 2°C - 8°C |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
M1386 Mag-Bind RXNPure Plus
SDS
M1386 SDS
APPLICATION NOTE
Optimized Magnetic Bead-Based DNA Clean Up with Low Elution Volumes on the Hamilton Microlab® STAR™
Product Data
High Recovery of Amplicons
Figure 1. 10 µL of 50 bp ladder was purified with Omega Bio-tek’s Mag-BIND® RXNPure Plus Kit and a comparable kit from Company A according to manufacturer’s recommended protocols. The DNA was eluted in 20 µL and analyzed on the Agilent TapeStation® 2200.
