PP2AC, Protein Phosphatase 2AC
Source: Bovine kidney
Purity: > 90% by SDS-PAGE, apparent Mr ~ 36-kDa
Supplied: 1 μg in 50 μl 50 mM Tris-HCl pH 7.0 buffer containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 50% glycerol.
Activity: ~ 2000 units/mg with myelin basic protein (MBP) as substrate. One unit is the amount of PP2AC that releases 1 nmol of inorganic phosphate from 32P-labeled MBP per min. Maintain preparations in aliquots at -70° C. Avoid repeated thawing.
Synonyms: Protein Phosphatase 2AC; Catalytic Subunit of Protein Phosphatase 2A; PP2AC
Background: Protein phosphatase 2A (PP2A) is a growth and tumor suppressor. It is a multifunctional divalent cation-independent protein serine/threonine phosphatase involved in regulating numerous cellular processes, including the cell cycle, growth and differentiation. Physiological targets of PP2A include cell surface receptors and ion channels, and protein kinases involved in mitogenic signaling and the cell cycle. They also include transcription factors and key regulatory enzymes involved in metabolism. The tumor promoters okadaic acid is a potent inhibitor of PP2A. The enzyme is inhibited also by I1PP2A and I2PP2A, two cancer-associated cellular proteins. The SV40 small t antigen replaces the B subunit during viral transformation and subverts the physiological function of a subset of PP2A complexes in a substrate selective manner.
Figure: SDS-PAGE pattern of purified preparation of PP2AC. The gel was stained with silver.
GloboZymes provides divalent cation-independent preparations of the catalytic subunit of PP2A (PP2AC). Among other uses, these preparations are used to study of enzyme kinetics and regulation. They are also used to dephosphorylate target substrates, and to evaluate the effects of test substances on the activity of the phosphatase. The preparations can also be used for studies on the role of the missing A/PR65 and variable B subunits of native forms of the enzyme e.g., PP2A1 and PP2A2.
References: Biochem J 287: 1019; Proc Natl Acad Sci USA 90: 2500
PP2A1, PP2A1, PP2A1, PP2A1, PP2A1, PP2A1, PP2A1, PP2A1, PP2A1, PP2A1,
