ESIBIO/ESI-035人胚胎干细胞系/≥ 5 x 10e5电池/ES-701

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货号:ES-701
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Morphology of ESI-035 at passage 18 in feeder-free culture.Zoom

  • Morphology of ESI-035 at passage 18 in feeder-free culture.
  • Karyogram of ESI-035 at passage 18 displays a normal karyotype (46, XX).
  • STR profile of ESI-035.

ESI-035 Human Embryonic Stem Cell Line

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Research grade hESC line derived from cGMP compliant banks grown in feeder-free culture conditions.
SKU: ES-701
$950.00

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T: 877.636.4978 E: orders@esibio.com

F: 510.648.3255

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Description

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ESI BIO offers the highest quality human embryonic stem cell (hESC) lines that are among the best-characterized and documented lines commercially available today. The ESI cell lines are NIH registered and backed with donor history and testing information in best compliance with current Good Tissue Practice (cGTP) and conform to Global Ethical Standards and Clinical Cell Regulations. ESI hESCs were derived under current Good Manufacturing Practice (cGMP) conditions on human fibroblast feeder layers and are available in both research and clinical-grade formats. For most investigators, research banks of the ESI hESC lines provide a convenient entry point, while GMP compliant banks of these same cells create seamless translation into clinically relevant studies.The ESI hESCs are well characterized in regards to genomic integrity, pluripotency status, and culturing behavior. Each line has been karyotyped, STR fingerprinted, has high viability thawing recovery, is characterized by pluripotency marker expression, and is free of mycoplasma, pathogens, bacteria, yeast, and fungus. In addition to the standard pluripotency and karyotype analysis, the complete genome sequence is available for purchase.
NIH registered lines

Details

Details

FormatResearch grade
Culture ConditionsFeeder independent in defined medium
Size≥ 5 x 10e5 cells
Passage Number<>
StorageLiquid nitrogen
Expanded and Banked byBioTime, Inc.
Karyotype46, XX
Positive Stem Cell MarkersOct4, Sox2, TRA-1-60, TRA-1-81
Technical Documents

ESI-035 Technical Data Sheet

Product Support

EDTA-based Passaging of ESI Human Embryonic Stem Cells Protocol

Brochures

Product Information Sheet

ReferencesNIH Human Embryonic Stem Cell Registry [Number 0129] »Crook, J.M., et al. (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1: 490-494. PMID: 18938745.Funk, W.D., et al. (2012) Evaluating the genomic and sequence integrity of human ES cell lines; comparison to normal genomes. Stem Cell Res 8(2): 154-164. PMID: 22265736.
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ESIBIO人类胚胎干细胞(hESC)系是根据当前的良好生产规范(cGMP)条件在人类成纤维细胞饲养层上衍生的,可用于研究和临床级格式。作为最早的临床级hESC系,ESI细胞系是当今可用的最佳特征和有据可查的,具有完整的基因组序列,STR指纹图谱和HLA型数据。ESI细胞系的生产符合质量体系,并以最符合当前的良好组织规范(cGTP)的捐赠者历史和测试信息为后盾,并符合全球伦理标准和临床细胞法规。完整的基因组概况和全面的数据可供购买由NIH批准,可在NIH干细胞注册处获得可在CIRM合作伙伴关系下获得资金