Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.

Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables rapid and crude isolation of of intact mitochondria from cultured mammalian cells. The kit offers two protocols for the separation of mitochondria from cytosolic components relying on differential centrifugation. First protocol utilizes a reagent-based method allowing multiple samples to be processed concurrently. The second protocol uses traditional Dounce homogenization and provides approximately two-fold more mitochondria.