品牌分类
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
- Datasheet
- Product description
- Publications & Images
- Related products
| Product Code | opr0031 |
|---|---|
| Uniprot ID | P49221 |
| Name | Transglutaminase-4, TGase-4 |
| Appearance | White lyophilized solid |
|---|---|
| Constituents | The TG is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5mM DTT, 1mM EDTASample contains maltodextrin |
| Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawingDelivery at ambient temperature is possible |
| Description | His6-rhTG4 is based on clone IMAGp958A10818Q2. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEELLL…. His6-rhTG4 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity. |
| Produced in | Escherichia coli |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7047 |
| Labelling | His-Tag |
| Preparation method | Add 10 mM Ca2+ to activate His6-rhTG4. |
| Molecular weight | 78 kDa |
| Principle | His6-rhTG4 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG4 may also be used for immunoprecipitation. |
| Purity | > 95 % |
| Recommendations | Add the volume of H2O to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage. |
| Product type | Proteins |
| Applications | Immunoprecipitation, Functional Assay |
| Research areas | Transglutaminase |
