- Cell Technology_Cell Technology
- Cell Signaling Technology(CST)抗体的亲情包装和防伪码,购买认准...
- 朗文教育喜迎开学季 金秋钜惠 震撼来袭!在线订票互动吧
- celltechnology|产品目录
- Cell Technology 品牌 代理
- 玉博|celltechnology公司|产品资讯|蚂蚁淘进口试剂平行进口
- 斗鱼 每个人的直播平台
- 斗鱼 每个人的直播平台
- Cell TechnologyELISA 试剂盒
- NBS1缺 失突变导致小颅畸形的分子机制分析
- 求加精： 进口品牌清关代理,有谁知道吗？Cellgs品牌咨询
- ...in the very low density lipoproteins of human plasma.
- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Introduction
The two new novel probes, Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) developed by Tetsuo Nagano et. al. (1), are selective for the detection of highly reactive oxygen species (hROS). Both probes have little reactivity towards other ROS such as: singlet oxygen (021), superoxide (O2-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1. HPF/APF are cell permeable and can be used in combination to detect hypochlorite (-OCl) production in cells (see fig 1). Hypochlorite can be detected by loading two samples, one with APF and the other with HPF. Hypochlorite production is visualized by increase in fluorescence of APF loaded cells and no increase in fluorescence in HPF loaded cells.
Key Benefits
- Quenched Cell permeable dye.
- One Step, No wash Homogenous assay.
- Adaptable to High throughput assay platforms.
- Can monitor multiple time points to follow real time kinetics.
- Non-destructive cell based assay allows monitoring of additional parameters.
- Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.
Additional information
| Kit Size | 100 |
|---|
Figure : Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters1. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.
| Document Title |
| OclProtocol |
| OCI Datasheet |
| msds.APF-HPF-OCl |
| Reference |
| Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003 |
| Part# | Reagent | Temperature |
| Part# 4012 | HPF Dye, 1 Vial | 2-8C |
| Part# 4011 | APF Dye, 1 Vial | 2-8C |
