Biocare’sPromARK™seriesintroducesMouse-on-CanineAP-PolymerDetection.Thisdetectionisspeciallydesignedforusingmouseantibodiesoncanineandfelinetissuesandutilizesbiotin-freealkalinephosphatase(AP)polymertechnology.Theadvantagesofthispolymertechnologyareincreasedsensitivity,reductionofIHCsteps(noAvidin/BiotinblockorLink/Probe),andminimalcross-reactivitytoendogenouscanineandfelineIgG.TheMouse-on-Caninedetectioncanbeusedwithparaffin-embeddedtissuesandcanbeperformedmanuallyoronautomatedstainingplatforms.TheadditionofproprietaryblockerstothedetectionreagentpermitstheuseofanyofBiocareMedical’sretrievalsolutions(Reveal/Borg/Diva)orenzymeswiththeeliminationofendogenousIgGornon-specificbackgroundstaining.TemperaturedependentprotocolscanbeconvenientlyperformedusingBiocare’sDecloakingChamber.TheMouse-on-CanineAP-PolymermaybecombinedwiththeRabbit-on-CanineHRP-PolymertocreateadoublestainpolymerdetectionthatwilllabelthemouseantibodywithAPandtherabbitantibodywithHRP.RefertoTechnicalNotes#5and#6foradditionalinformation.
