Cytotoxiclymphocytes,includingNKcellsandcytotoxicTlymphocytes(CTLs),playamajorroleinthedefenseagainstneoplasticprocessesandviralinfections(1).Granuleexocytosisisthemechanismbywhichcytotoxiclymphocytesmayinducelysisofitstarget.Thisinvolvesgranule-associatedcytotoxicproteins,includingtheT-cellintracellularantigen-1(TIA-1),perforinandgranzymeB(1,2).PerforinproducesporesthatallowtheentryofothercytotoxicproteinssuchasTIA-1andgranzymes,whichtriggeraprocessleADIngtoDNAfragmentationandapoptosisofthetargetcells(1).However,thereisevidencethatgranzymeBmaykilltargetsindependentlyofperforin(2).Recentstudies(3,4,5)havedemonstratedthattheexpressionofgranzymeBbyimmunohistochemistryinseveralentitiesofextranodalperipheralTcelllymphoma(PTCL)andNKcelllymphomas,includingnasalandnasal-typeNK/Tcelllymphomas,hepatosplenicandnon-hepatosplenicPTCL,enteropathy-type(ETCL)andnon-ETCLintestinalPTCL,subcutaneouspanniculitis-likePTCL(SPTCL),cutaneousCD8+epidermotropiclymphomasaswellasinnodalandcutaneousCD30+anaplasticlargecelllymphomas(ALCL)(3,4,5).Incontrast,onlyafewnodalPTCL-unspecified(UC)expresscytotoxicphenotypewhileangioimmunoblastic(AILD)lymphomasdonot(3,4,5).Theexpressionofcytotoxicproteins,suchasgranzymeBmaybeimportantfortheidentificationandclassificationofextranodalT-andNK-celllymphomassincemanyofthesetumorsdonothavespecificmorphologyandphenotype(6,7).
| Format | Concentrate,Predilute |
|---|---|
| Volume | 0.1ml,0.5ml,6.0ml |
| SpeciesReactivity | Human;othersnottested |
| Clone | 11F1 |
| IntendedUse | IVD |
| Localization | CytoplasmicLyticGranules |
| Antigen | RecombinantproteincorrespondingtotheNterminusofgranzymeB |
| Isotype | IgG2a |
| DownloadDataSheet |
| DownloadSDSSheet |
References:
1.SmythMJ,TrapaniJA.Granzymes:exogenousproteinasesthatinducetargetcellapoptosis.lmmunolToday.1995Apr;16(4):202-6.
2.FroelichCJ,DixitVM,YangX.Lymphocytegranule-mediatedapoptosis:mattersofviralmimicryanddeadlyproteases.ImmunolToday.1998Jan;19(1):30-6.
3.KrenacsL,etal.CytotoxiccellantigenexpressioninanaplasticlargecelllymphomasofT-andnull-celltypeandHodgkin’sdisease:evidencefordistinctcellularorigin.Blood.1997Feb1;89(3):980-9.
4.KanavarosP,etal.Cytotoxicproteinexpressioninnon-Hodgkin’slymphomaandHodgkin’sdisease.AnticancerRes.1999Mar-Apr;19(2A):1209-16.
5.KanavarosP,etal.ExpressionofcytotoxicproteinsinperipheralTcellandnaturalkiller-cell(NK)lymphomas:associationwithextranodalsite,NKorTgammadeltaphenotype,anaplasticmorphologyandCD30expression.LeukLymphoma.2000Jul;38(3-4):317-26.
6.JaffeES,etal.ExtranodalperipheralT-cellandNK-cellneoplasms.AmJClinPathol.1999Jan;111(1Suppl1):S46-55.Review.
7.KinneyMC.Theroleofmorphologicfeatures,phenotype,genotypeandanatomicsiteindefiningextranodalT-cellandNK-cellneoplasms.AmJClinPathol.1999Jan;111(1Suppl1):S104-18.
8.CenterforDiseaseControlManual.Guide:SafetyManagement,NO.CDC-22,Atlanta,GA.April30,1976“DecontaminationofLaboratorySinkDrainstoRemoveAzideSalts.”
9.ClinicalandLaboratoryStandardsInstitute(CLSI).ProtectionofLaboratoryWorkersfromOccupationallyAcquiredInfections;ApprovedGuideline-FourthEditionCLSIdocumentM29-A4Wayne,PA2014.
