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DenaturingSolutionisusedasanelutionstepwhenusingadoublestaintechnique(Biocare’s7-stepdoublestainprocedure).Thiswillensurethatthesecondstainingprotocolwillnotcrossreactwiththefirststainingprotocol.SolutionA(yellow)isaconcentratethatmustbedilutedwithSolutionB(red,dilutionbuffer)forproperelution.ItisrecommendedthattheDenaturingSolutionbeusedatdifferentdilutionsdependingontissuefixationandthetypeofantigenretrievalmethodused.
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