Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi (NP Protein, N Protein)

Source: recombinant SARS-CoV-2 Nucleocapsid protein (Ser2-Ala419) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: QHD43416.1Predicted molecular mass: 48.9 kDa.Endotoxin: Less than 1 EU per ug by the LAL method.Formulation: The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid protein-His-Avi is supplied as 0.22 um filtered solution in 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5.Purity: > 95% by PAGE under reduced condition.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.

Background

The virus causing Coronavirus Disease 2019 (COVID-19) was named 2019 novel coronavirus (2019-nCoV) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Similar to those of other coronaviruses, there are four major structural proteins in SARS-CoV-2, S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The main function of Nucleocapsid protein is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells.