BIO is a cell-permeable, potent, reversible and ATP-competitive GSK-3α/β inhibitor (IC50 = 5 nM). Specificity was confirmed using various Cdk"s (IC50 = 83 nM for Cdk5/p25, 300 nM for Cdk2/cyclin A, 320 nM for Cdk1/cyclin B, and 10 µM for Cdk4/cyclin D1), MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK (IC50 ≥ 10 µM).BIO is the first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells. Human embryonic stem cells (hESCs) are maintained in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition.
CAS Number
667463-62-9
Chemical Formula
C16H10BrN3O2
Chemical Name
6-bromoindirubin-3′-oxime
Molecular Weight
356.17
QC Specifications
MASS Spec
Mouse ESC culture for toxicity
Shipping Information
on blue ice
Storage
4°C protected from light
Stability
Stable for up to 6 months from date of receipt when stored as directed.
Reference
1. Polychronopoulos, P., et al. 2004. J. Med. Chem. 47, 935.
2. Sato, N., et al. 2004. Nat. Med. 10, 55.
3. Meijer, L., et al. 2003. Chem. Biol. 10, 1255.
