生物测定系统/QuantiFluo™Caspase-3检测试剂盒/100次检测/DCS3-100

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¥4580.00
货号:DCS3-100
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品牌:BioAssay Systems
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商品描述

QuantiFluo™ Caspase-3 Assay Kit

QuantiFluo™ Caspase-3 Assay Kit Catalog No: DCS3-100
Price: $229 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: RT Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
Caspase Assay Kit
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ProtocolSDS

Application

  • For quantitative determination of the apoptosis target caspase-3 activity and HTS screen for apoptosis inducers and inhibitors.

Key Features

  • Safe. Non-radioactive assay.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • FL400/490nm

Samples

  • Cell and tissue lysate

Species

  • All

Size

  • 100 tests

Shelf Life

  • 12 months

More Details

  • Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction. BioAssay Systems Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product.The fluorescence intensity (λex/em = 400/490nm) is proportional to the caspase-3 activity.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems酸性磷酸酶(ACP)是一种在消化过程中催化磷酸基团从其他分子上裂解的酶。酸性磷酸酶可以在溶酶体中发现,并在与内体融合,酸化pH值后变得有活性,从而为ACP创造了最佳环境。ACP还可以在骨骼,脾脏,肝脏,肾脏和血液中发现。血清水平可以用作前列腺癌的生物标志物,尽管前列腺特异性抗原(PSA)被更广泛地使用。BioAssay Systems非放射性比色ACP分析基于从合成底物上裂解对硝基苯酚。加入终止剂后,对硝基苯酚会显色。加入终止剂后在405 nm处吸光度的增加与酶活性成正比。