Make direct quantitative measurements in biological samples
- Gas permeable polymer membrane sleeve blocks liquids, ions and particulate matter
- Incorporated reference electrode
- For use with Apollo1000, Apollo4000, TBR4100 and TBR1025
- Response Time: < 10s
- Detection Limit: 0.1% to 100%
- Drift: <1%/min
- Package of 1
Benefits
- Clark type of oxygen sensor with tip size of 2 mm, can be used for very small volume O2 measurement
Applications
- Cell culture, cell suspensions
- Cell tissue measurements
This sensor incorporates WPI’s proprietary combination electrode technology whereby the oxygen-sensing element and separate reference electrode are encased within a single shielded sensor design. A gas-permeable polymer membrane is fitted over the end of the sleeve, which allows oxygen to pass while blocking liquids, ions and particulate matter.
Oxygen diffuses through the membrane. The voltage applied to the sensor is held at -0.7V when the monitoring device is on and the sensor is properly connected. The magnitude of the generated electrical current is determined by the rate of diffusion through the membrane. The rate is proportional to the partial pressure of oxygen outside the membrane. The current serves as a measure of the partial pressure of O2.
The ISO-OXY-2 is a 2.0 mm stainless steel sensor, with replaceable membrane sleeves (5378) and an internal refillable electrolyte (#7326).
NOTE: The 2mm biosensors come with two spare membrane sleeves, 1cc syringe, an MF28G67 MicroFil needle and 10mL of filling solution (#7326).
ISO-OXY-2 Instruction Manual
| APPLICATION | Cell cultures, cell suspensions |
| SENSOR DIAMETER | 2.0 mm |
| RESPONSE TIME | <10 SEC (90%) |
| DETECTION LIMIT | 0.1 % to 100% |
| DRIFT | <1%/min |
| SENSITIVITY | N/A |
| PHYSIOLOGICAL INTERFERENCE | None |
X. Liu, C. Cheng, N. Zorko, S. Cronin, Y.R. Chen, J.L. Zweier "Biphasic modulationof vascular nitric oxide catabolism by oxygen" Am J Phhsiol Heart circ Physiol 287. 2004: H2421-H2426
Olson, K. R., Gao, Y., DeLeon, E. R., Arif, M., Arif, F., Arora, N., & Straub, K. D. (2017). Catalase as a sulfide-sulfur oxido-reductase: An ancient (and modern?) regulator of reactive sulfur species (RSS). Redox Biology, 12, 325–339. https://doi.org/10.1016/j.redox.2017.02.021
Hirai, T., Osamura, T., Ishii, M., & Arai, H. (2016). Expression of multiple cbb3 cytochrome c oxidase isoforms by combinations of multiple isosubunits in Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America, 113(45), 12815–12819. https://doi.org/10.1073/pnas.1613308113
Park, Y. M., Lee, H. J., Jeong, J.-H., Kook, J.-K., Choy, H. E., Hahn, T.-W., & Bang, I. S. (2015). Branched-chain amino acid supplementation promotes aerobic growth of Salmonella Typhimurium under nitrosative stress conditions. Archives of Microbiology, 197(10), 1117–1127. https://doi.org/10.1007/s00203-015-1151-y
Universal Dipping Probe Stand42200For pricing, Customers outside of the US and Canada, please contact your distributor.
Start-up Kit forISO-OXY-2 or OXELP5377For pricing, Customers outside of the US and Canada, please contact your distributor.
ISO-OXY-2 or OXELP Sleeve Kit5378For pricing, Customers outside of the US and Canada, please contact your distributor.
ISO-OXY-2 or OXELP Filling Solution7326For pricing, Customers outside of the US and Canada, please contact your distributor.
TBR PBS Calibration SolutionTBR-PBS-CALFor pricing, Customers outside of the US and Canada, please contact your distributor.
