PCI – Protein C Inhibitor Polyclonal Antibody – Affinity Purified
GAPCI-AP – Goat anti human PCI – Protein C Inhibitor , affinity purified IgG (0.5 mg vial)
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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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https://www.ebiomall.com
商品描述
Description
Protein C Inhibitor Deficient Plasma
Protein C Inhibitor Deficient Plasma is manufactured from normal citrated human plasma that has been depleted of Protein C Inhibitor Deficient Plasma by selective affinity immuno-adsorption using antibodies directed towards Protein C Inhibitor. Available in two different formats – Frozen and Lyophilized
Only the highest quality citrated plasma is used as starting material and in many cases the parent plasma is available as control material. Our Protein C Inhibitor Deficient Plasma can be used for further manufacturing or research use only applications.
Frozen PCI Deficient Plasma
Product Code: PCI-DP
Presentation: Frozen Protein C Inhibitor Deficient Plasma
Preparation/Handling: Thaw 1 ml vials in 37oC water bath for 5 minutes; for bulk volumes, thawing time will be dependent on bottle size.
Storage and Stability: Plasma is shipped frozen and should be stored below -60oC. Product is stable until date stated on vial label when stored at -60oC. Once thawed, plasma is stable for 4 hours at 2-8oC in original vial.
Certificate of Analysis: available upon request
Lyophilized PCI Deficient Plasma
Product Code: PCI-LDP
Presentation: Freeze Dried Protein C Inhibitor Deficient Plasma containing 50 mM HEPES and stabilizers.
Reconstitution: Reconstitute with 1 mL of reagent grade water. Allow contents to dissolve for 15 minutes at room temperature with occasional swirling. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Storage and Stability: Prior to reconstitution, dried plasma should be stored at 2-8°C. Product is stable until date stated on vial label when stored at 2-8oC. Stability after reconstitution is 4 hours at ambient (18-25oC), or 30 days at –20oC.
Certificate of Analysis: available upon request
Description of Protein C Inhibitor (PCI)
Protein C Inhibitor (PCI), also known as Plasminogen Activator Inhibitor 3 (PAI3), is a member of the SERPIN family of proteinase inhibitors. It is produced in the liver as a single chain glycoprotein (mass of 57 kDa) and circulates in plasma at a concentration of 5 μg/ml (~90 nM). PCI is also found in urine in lower concentrations of 250 ng/mL (~0.4 nM). PCI is the primary inhibitor of activated Protein C (APC) in plasma but demonstrates a relatively broad specificity, also inhibiting thrombin, FXa, FXIa, kallikrein, tPA, urokinase, prostate specific antigen, acrosin, chymotrypsin and trypsin. The preferred enzyme target for PCI appears to be thrombin and this interaction is increased by more than 100 fold in the presence of thrombomodulin. Like ATIII and HCII, the inhibitory activity of PCI towards some of these enzymes is stimulated by high concentrations of heparin (5 U/ml) which can accelerate the rate of inactivation as much as 50 fold. Enzyme inhibition by PCI occurs through proteolytic cleavage at Arg354-Ser355 and subsequent rapid formation of a stable, inactive 1:1 enzyme-PCI complex. Interaction with APC results in an SDS-stable APC-PCI complex of 102 kDa.1-4.
References and Reviews
- Suzuki K; Protein C Inhibitor; Methods in Enzymology 222, pp 385-399, 1993.
- Cooper ST, Whinna HC, Jackson TP, Boyd JM, Church FC; Intermolecular Interactions between Protein C Inhibitor and Coagulation Proteases; Biochemistry: 34, pp. 12991-12997, 1995.
- Heeb MJ, Espana F, Geiger M, Collen D, Stump DC, Griffin JH; Immunological Identity of Heparin-dependent Plasma & Urinary Protein C Inhibitor & Plasminogen Activator Inhibitor-3; JBC:262, pp 15813-15816, 1987.
4. Rezaie AR, Copper ST, Church FC, Esmon CT; Protein C Inhibitor is a Potent Inhibitor of the Thrombin-Thrombomodulin Complex; JBC:270, pp 25336-25339, 1995.
Related Products:
PCI – Protein C Inhibitor Polyclonal Antibody – HRP Conjugated
GAPCI-HRP – Goat anti human PCI – Protein C Inhibitor , peroxidase conjugated IgG (0.2 mg vial)
Protein C Inhibitor Deficient Plasma
PCI-DP – Frozen Protein C Inhibitor Deficient Plasma – 1ml or bulk quantitiesPCI-LDP – Lyophilized Protein C Inhibitor Deficient Plasma – 1ml
