- SynonymNTB-A,SLAMF6,Ly108,NK-T-B-antigen,CD352,KALI
- SourceBiotinylated Human NTB-A, His,Avitag (NTA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Met 226 (Accession # Q96DU3-1).Predicted N-terminus: Gln 22Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 26.8 kDa. The protein migrates as 35-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human NTB-A, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundThe SLAM family of receptors is composed of six members named SLAM (CD150); 2B4 (CD244); Ly-9 (CD229); CD84; natural killer, T and B cell antigen (NTB-A; Ly108 in the mouse. Most, if not all,immune cell types express more than one SLAM family receptor. All six SLAM-related genes are located within a 400–500 kilobase (kb) genomic segment on chromosome 1 in humans and mice. This feature implies that the genes that encode the SLAM family were created by serial gene duplication.
- References
- (1)Veillette A., et al. 2007, Immunity., 27(5), 698-710.
- (2)Detre C., et al. 2010, Semin Immunopathol., 32(2), 157-71.
- (3)Nakajima H., et al. 2010, Semin Immunopathol., 32(2), 157-71.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
