- SynonymNSP7,nsp7,Non-structural protein 7,COVID-19
- SourceSARS-CoV-2 NSP7, His Tag (NS7-C51H6) is expressed from E.coli cells. It contains AASer 1 - Gln 83 (Accession # YP_009725303.1).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 11.3 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Delivered as bulk protein in a 0.2 μm filtered solution of PBS, pH7.4 with glycerol as protectant.
Contact us for customized product form or formulation.
- Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
- ShippingThis product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SARS-CoV-2 NSP7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 NSP7, His Tag (Cat. No. NS7-C51H6) was more than 90% and around 20-25 kDa verified by SEC-MALS.
- BackgroundDuring the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp"s. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
- References
- (1)Krichel B, et al. Biochem J. 2020. 477(5):1009-1019.
- (2)Kirchdoerfer RN, et al. Nat Commun. 2019. 10(1):2342.
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