- SynonymMMP2,CLG4,CLG4A,MMP-II,MONA,TBE-1
- SourceHuman MMP-2, His Tag (MM2-H5225) is expressed from human 293 cells (HEK293). It contains AA Ala 30 - Cys 660 (Accession # AAH02576).Predicted N-terminus: Ala 30Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 71.8 kDa. The protein migrates as 66-71 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>92% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human MMP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
- Citations
Tumor-responsive small molecule self-assembled nanosystem for simultaneous fluorescence imaging and chemotherapy of lung cancer
Authors: Yang Y, et al.
Journal: Adv Funct Mater 2016
Application: Cell Culture
Request for Full-text
Multifunctional thermoresponsive designer peptide hydrogels
Authors: De Leon-Rodriguez LM, et al.
Journal: Acta Biomater 2016
Application: Assay for Enzyme Activity
Request for Full-text
- BackgroundMatrix metalloproteinase-2 (MMP-2) is also known as 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A and CLG4A, which belongs to the peptidase M10A family. MMP-2 / CLG4A contains 3 fibronectin type-II domains and 4 hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. MMP-2 cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. MMP2 involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin.
- References
- (1)Brooks P.C., et al., 1998, Cell 92:391-400.
- (2)Fernandez-Patron C., et al., 1999, Circ. Res. 85:906-911.
- (3)Fernandez-Patron C., et al., 2000, Circ. Res. 87:670-676.
- (4)Bello L., et al., 2001, Cancer Res. 61:8730-8736.
- (5)Lovett D.H., et al., 2012, PLoS ONE 7:E34177-E34177.
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