- SynonymEPHA7,EHK3,HEK11,hEK11
- SourceHuman EphA7, His Tag (EP7-H5221) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ile 556 (Accession # AAI26126).Predicted N-terminus: Gln 27Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.2 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>98% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human EphA7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
- BackgroundEphrin type-A receptor 7 (EPHA7) is also known as EPH homology kinase 3 (EHK3), EPH-like kinase 11 (HEK11), which belongs to the protein kinase superfamily or tyr protein kinase family or ephrin receptor subfamily. EPHA7 / EHK3 contains 1 Eph LBD (Eph ligand-binding) domain, 2 fibronectin type-III domains, 1 protein kinase domain, 1 SAM (sterile alpha motif) domain. EHK-3 / EPHA7 is widely expressed. The catalytic activity of EPHA7 / EHK3 is “ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate”. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
- References
- (1)Nakanishi H., et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104:14442-14447.
- (2)Tsuboi M., et al., 2010, Int. J. Oncol. 36:635-640.
- (3)Eph nomenclature committee, 1997, Cell 90:403-404.
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