嵌合体/碱性磷酸酶,E。大肠杆菌/1020/1000单位

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货号:1020
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品牌:Chimerx
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Source

Escherichiacoli

Applications
  • MorethermalstablethanCalfIntestineAlkalinePhosphatase(CIP)
  • Optimalincubationtemperatureisapproximately60°C,howevertheenzymeremainsactivefrom20°Cto80°C.
  • Resistanttochemicalchangesandactiveoverabroadrangeofbufferconditions.
  • Canbeusedtoremove5"-phosphatesfromDNAorRNApriorto5"-endlabeling(1).
  • Workstoremove5"-phosphatesfromlinearizedvectormoleculestopreventself-ligationofthevectorduringcloningprocedures(1).
  • Idealfordiagnosticimmunoassaysandimmunodetectionofproteinsandnucleicacidsfollowingblottingexperiments(1).
UnitDefinition

Oneunitistheamountofenzymerequiredtohydrolyze1molofp-nitrophenylphosphate37°Cforone(1)minuteinabufferconsistingof:

0.25mMMgCl2 (pH9.8).
1Mdiethanolamine.
10mMp-nitrophenylphosphate.

StorageBuffer

20mMTris-HCl(pH7.0at22°C)
5mMpotassiumphosphate
100mMKCl
0.1mMMgCl2
0.1mMZnCl2  
+StABIlizers

QualityControl

AllpreparationsareassayedforcontaminatingendonucleaseandnonspecificRNaseandsingle-anddouble-strandedDNaseactivities

StorageConditions

Storeat-20°C
Productshippedondryice

References

(1)Sambrook,J.etal.(1989)Molecularcloning:AlaboratoryManual,secondedition,pp.1.56,5.72ColdSpringHarbor,NewYork

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