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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
RsrIISource
Rhodopseudomonassphaeroides
ReagentsSupplied
CMXBuffer4
UnitDefinition
Oneunitistheamountofenzymerequiredtodigest1µgoflamBDaDNAwith≥95%efficiencyin1hourinatotalreactionvolumeof50μl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer4:
20mMTris-Acetate(pH7.5at37ºC)
50mMPotassiumAcetate
10mMMagnesiumAcetate
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1:50%
Buffer2: 75%
Buffer3:25%
Buffer4:100%
Buffer5:50%
StorageBuffer
10mMTris‑HCl(pH7.5at4ºC)
0.1mMEDTA
50mMKCl
1mMdithiothreitol
200µg/mlbovineserumalbumin
50%(v/v)glycerol
AssayConditions
20mMTris‑Acetate(pH7.5at37ºC)
50mMPotassiumAcetate
10mMMagnesiumAcetate
1.0mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
Notes
- LambdaDNAcontains3slow-cleavagesitesthatdonotcompletelydigestafter1hourincubation.
- Completedigestionofthesesitesisachievedby4UnitsofRsrIIwith1μgoflambdaDNAincubatedat37°Cfor16hours.
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases
