Details
Nco I Source
Nocardia corallina
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 75%
Buffer 2: 75%
Buffer 3: 75%
Buffer 4: 100%
Buffer 5: 100%
*Star activity
Storage Buffer
50 mM Tris-HCl (pH 7.5 at 4°C)
100 mM KCl
1 mM EDTA
10 mM BME
500 µg/ml bovine serum albumin
0.1 mM PMSF
0.1% Triton X-100™
50% (v/v) glycerol
Assay Conditions
20 mM Tris Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA
Storage Conditions
Store at -20°C
Shipped on dry ice
Downloads
Certificate of Analysis PDF - Current Lot
MSDS PDF -Current Lot
Categories
Restriction Endonucleases
