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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
ReagentsSupplied
10XTthDNAPolymeraseReactionBuffer
Source
ThermusThermophilus
Applications
- Ccanreversetranscribeatelevatedtemperatures
- MinimizesproblemswithstrongsecondarystructureofRNA
- Resultsingreaterspecificityofprimerhybridizationandextension
- SuitableforhightemperaturesynthesisofDNA
- SynthesizesCDNAfromRNAtemplate
- StablethermophilicDNApolymerase(1)
UnitDefinition
Oneunitistheamountofenzymerequiredtoincorporate10nmolestotalnucleotideintoacidinsolublematerialat70°Cfor30minutes.
Concentration
5-10units/µl
StorageBuffer
50mMTrisHCl(pH7.5)
0.1mMEDTA
5.0mMdithiothreitol
50%(v/v)glycerol
StABIlizers
AssayConditions
25mMTris-HCl(pH9.5at25°C)
50mMpotassiumchloride
10mMmagnesiumchloride
1mMdithiothreitol
0.3µg/μlactivatedcalfthymusDNA
1μg/μl bovineserumalbumin
0.2mMofeachdCTP,dGTP,dTTPand[α-32P]dATP
Incubationisfor10minutesat70°C inatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Productshippedondryice
References
(1)Wang,A.M.,Doyle,M.V.,andMark,D.F.(1989)Proc.Nat’l.Acad.Sci.USA86,9717-9721
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
Polymerases,MesophilicDNAPolymerases
