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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Details
Source
bacteriophageT7of Escherichiacoli
Description
- DNA-dependentRNApolymerasewhichhasstringentspecificityforT7phagepromoterssequence(1)
- Ultrapurerecombinantenzyme
Applications
- EfficientlysynthesizesinvitrotranscriptsfromalmostanyDNAthatisdownstreamfromaT7promoter(2)
- Suitableforpreparinglabeledsingle-strandedRNAprobesofhighspecificactivity(3)
- Transcriptscanbeusedashybridizationprobes,templatesforinvitrotranslation,substratesinRNAprocessingsystems,orexonandintronmappingofgenomicDNA
ReagentsSupplied
10XT7RNAPolymeraseReactionBuffer
UnitDefinition
Oneunitistheamountofenzymerequiredtoincorporate1nmoloflabeledUTPintoacidinsolublematerialfor60minutes at37°C
Concentration
20-25Units/µl
StorageBuffer
20mMpotassiumphosphate(pH7.5)
100mMNaCl
1mMdithiothreitol
1mMEDTA
50%(w/v)glycerol
AssayConditions
40mMTris-HCl(pH7.9)
8mMMgCl2
10mMdithiothreitol
4mMspermidine(HCl)3
1ugpGEM-7Zf(+)plasmid
0.4mMeachofATP,CTP,GTPand[α-32P]UTP
Reactionvolumeis25µl
StorageConditions
Storeat-20°C
ShippedonDryIce
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF
References
(1)Chamberlin,M.andRing,J.(1973)J.Biol.Chem.248,2235-2244
(2)Tabor,S.andRichardson,C.C.(1985)Proc.Natl.Acad.Sci.USA82,1074-1078
(3)Sambrook,J.,Fritsch,E.F.andManiatis,T.(1989)Mol.Cloning:ALab.Manual,SecondEd.,pp.10.27-10.37
