주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
□ 제품설명
Inorganic pyrophosphate (also referred toas diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number ofdifferent enzymatic reactions. Enzymes that utilize PPi as a substrate mayinclude phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymesthat generate PPi are more numerous and may include cyclases (EC 4.6.1),hydrolyses (EC 3) and ligases (EC 6). The PPiLight™ Assay is a non-radioactivebioluminescent assay for the detection of inorganic pyrophosphate. In thepresence of PPi the detection reagent catalyses the conversion of AMP to ATP.The assay uses luciferase, which produces light from the newly formed ATP andluciferin.
Figure 1: Bioluminescent reaction
□ 특장점
● Fast - Measure enzyme activityvia pyrophosphate consumption in 1 hour
● Simple - The 2 step,luminescent assay doesn’t require radioactive substrates,
beads, detectionantibodies or modified substrates
● Wide Detection Range - Sensitiveto 0.02 μM with a linear range to 10 μM
● Versatile - Scalable to 96-,384-, 1586-well formats
□ Applications
Measure activity of:
● Phosphotransferases
● Pyrophosphotases
● Cyclases
● Ligases
● Hydrolases
● DNA Polymerases
□ 보존
2°C - 8°C
